DAVID TILTON DENHARDT

Curriculum Vitae and Resumé

 

 

March 30, 2011

DAVID TILTON DENHARDT 

Curriculum Vitae and Resumé

Academic experience:

B.A. 1960        Swarthmore College, Swarthmore, Pennsylvania.  In Chemistry, with High Honors.

Ph.D. 1965      California Institute of Technology, Pasadena, California.  In Biophysics, with a minor in Physics.  Doctoral Thesis: "A Biophysical Study of Bacteriophage φX174 Replication" Graduate Supervisor:   Robert L. Sinsheimer.

1964-1966       Instructor in Biology, Harvard University, Cambridge, Massachusetts.

1966-1970       Assistant Professor of Biology, Harvard University, Cambridge, Massachusetts.

1970-1977       Associate Professor of Biochemistry, McGill University, Montreal, Quebec. 

1977-1980       Professor of Biochemistry, McGill University, Montreal, Quebec.

1980-1988       Professor of Biochemistry, Professor of Microbiology and Immunology, and Director of the Cancer Research Laboratory, University of Western Ontario, London, Ontario.  (1982-1988, Professor, part time, Department of Radiation Oncology.)

1988-1996       Professor II, Department Biological Sciences, Rutgers University, New Brunswick, NJ.

1988-1995       Chairman, Department of Biological Sciences, Rutgers University.

1988-1995       Director, Bureau of Biological Research, Rutgers University.

1991-1994       Director, Graduate Program in Cell & Developmental Biology, Rutgers University.

1996 - 2010     Professor II, Department of Cell Biology and Neuroscience, Rutgers University.

2010 -              Emeritus Professor of Cell Biology, Rutgers University

 

Membership (present or past) in the following Honorary and Professional Societies:

Phi Beta Kappa, Sigma Xi

American Society for Biochemistry and Molecular Biology

American Society for Microbiology

American Association for Cancer Research

American Society for Cell Biology

New York Academy of Sciences

Fellow, The Royal Society of Canada

Fellow, American Association for the Advancement of Science,

Fellow, American Academy of Microbiology

 

Address:          3 Wimple Way, Bridgewater, NJ 08807

                        Phone/Fax: 908-704-0279

Email:  denhardt@biology.rutgers.edu

                        http://lifesci.rutgers.edu/~denhardt/

 

Honors, Awards, Editorial Boards:

National Science Foundation Predoctoral Fellow, 1960-64.

Outstanding Young Men of America, 1971.

Research Grants from the National Science Foundation, United States Public Health Service, American Cancer Society, Medical Research Council of Canada, National Cancer Institute of Canada, Quebec Ministry of Education.  Membership on the Genetics Grants Panel of the Medical Research Council, 1972-1977, 1979-1983, Chairman, 1974-1977.

Member of the Fellowship Grants Panel of the National Cancer Institute, 1979-1982.

Listed in American Men and Women of Science, 12th Edition, and subsequent editions

Volume 2, 1972, Jacques Cattell Press, New York.

Who's Who in America, 39th Edition, and subsequent editions, Marquis Who's Who Inc.

Member of the Editorial Board of the Journal of Virology, 1975-1977.

Associate Editor, Virology, March 1975 - December, 1976.

Editor of the Journal of Virology, 1977-1987.            Editor, Gene 1984-1992.

Regional Editor, Journal of Cancer Research and Clinical Oncology, 1990 -

            Editor, Experimental Cell Research, 1994 -  2006

Associate Editor, Journal Cellular Biochemistry, 1994 –

Editorial Board Member, British Journal of Cancer, 1998 -

Various ad hoc NIH and Army Grants Panels

 

Books and Monographs:

Wald, G., Hopkins, J. III, Albersheim, P., Dowling, J. and Denhardt, D.T. (1966).  Twenty-six Afternoons of Biology, Addison-Wesley, Reading, Mass.

Denhardt, D.T., Dressler, D.H., Ray, D.S. (editors) The Single-stranded DNA Phages, Cold Spring Harbor Monograph, 1978.

Denhardt, D.T. (1982) Replication of DNA Carolina Biology Reader #120.  Edited by J.J. Head.  Published by Carolina Biological Supply Company, North Carolina.

Rodriguez, R., Denhardt, D. (editors) Vectors: A Survey of Molecular Cloning Vectors and Their Uses, Butterworth Publishers, 1987.

Baserga, R. and Denhardt, D.T. (editors) Antisense Strategies, Annals of the New York Academy of Sciences, Vol. 660, 1992.

Denhardt, D.T., Butler, W.T., Chambers, A.F., and Senger, D.R. (editors) Osteopontin: Role in Cell Signalling and Adhesion, Annals of the New York Academy of Sciences, Volume 760, 1995.

Stein, G.S., Baserga, R., Giordano, A., and Denhardt, D.T. (editors) The Molecular Basis of Cell Cycle  and Growth Control J. Wiley & Sons, New York and London. 1998

 

Chapters in Books:

A Theory of DNA Replication (J. Theoretical Biology 34: 487-580, 1972) was included in the series of Benchmark Papers in Microbiology, Vol. 3: Microbial Genetics, Edited by M.A. Abou-Sabe.  Dowden, Hutchison and Ross, Inc. 1973.

The Isometric Single-stranded DNA Phages.  Chapter I, Volume 7, in the series Comprehensive Virology, Edited by H. Fraenkel-Conrat and R.R. Wagner, Plenum Press (1977).

Changes in Gene Expression During Senescence/Immortalization of Mouse Embryo Fibroblasts.  In Growth Control During Cell Aging, Edited by R. Warner and E. Wang, CRC Press (1988).

Regulation of Gene Expression by the Tumor Promoter TPA.  In Genes and Signal Transduction in Multistage Carcinogenesis, Edited by N.H. Colburn, Alan R. Liss, Inc., N.Y. (1988).

Oncogenic Consequences of Down-modulating TIMP Expression in 3T3 Cells with Antisense RNA.  In Matrix Metalloproteinases and Inhibitors, Edited by H. Birkedal-Hansen, Z. Werb, H. Welgus, and H. van Wart.  Gustav Fischer Verlag, 1992.

Isolation of cDNA clones of low abundance mRNAs:  Alternative strategies in differential and subtractive hybridization, by R. Hofbauer and D. T. Denhardt. In: Methods in Molecular Genetics, edited by Kenneth W. Adolph, Academic Press, CA.  1993

Anticarcinogenic activities of naturally occurring cysteine proteinase inhibitors, by  R. Colella, A.F. Chambers, and D.T. Denhardt. In "Proteinase Inhibitors as Cancer Chemopreventive Agents" Edited by W. Troll and A. Kennedy.  Plenum Publishing Corp.  pp 199-216, 1993.

The Isometric Phages, by D. T. Denhardt In The Encyclopedia of Virology.  Edited by R.G. Webster and A. Granoff.  Academic Press Inc.  pp 989-996. 1994

On the Paradoxical Ability of TIMPs either to Inhibit or to Promote the Develop­ment of the Malignant Phenotype, by D.T. Denhardt In Inhibitors of Metalloproteinases in Development and Disease Edited by S.P. Hawkes, D.R. Edwards, and R. Khokha. Harwood Academic Publishing, Lausanne, Switzerland. 1997.

Signal transduction pathways and regulation of the mammalian cell cycle: Cell type-dependent integration of external signals, by D. T. Denhardt. In The Molecular Basis of Cell Cycle and Growth Control  (Editors: G. S. Stein, R. Baserga, A. Giordano, D. T. Denhardt)  J. Wiley & Sons Inc., New York and London, 1998.

Membrane Receptors and Signal Transduction Pathways in G1: Regulation of Liver Regeneration and T Cell Proliferation. By J. F. Porter and D. T. Denhardt. In Cell Cycle and Growth Control: Biomolecular Regulation and Cancer, Second Edition. (Editors G. S. Stein and A. B. Pardee). J. Wiley & Sons, 2004.

Metastasis: a current perspective. DT Denhardt, AF Chambers, DR Welch. Chapter 1 in Selected Aspects of Cancer Progression: Metastasis, Apoptosis and Immune Response pp1-10. Edited by HE Kaiser et al. 2008 Springer

 
 

Masters Theses Supervised

Xiaojia Guo                 ”Identification of 5B10, a serum-inducible mRNA, as Mouse Calcyclin: Expression and Characterization”.  University of Western Ontario, 1990.       

Yao Paul Zhang           “Characterization and Identification of Transcription Factors that Bind to a Novel Ras-activated Element”.  Rutgers University, 1995.

Devra S. Mistretta        “Systems for the Isolation of Genes Regulating and Regulated by Osteopontin: Differential Display and the Yeast One-Hybrid System”. Rutgers University, 1999

Richard Adamo           “Osteopontin as an Inhibitor of iNOS Expression in Cytokine-Stimulated Mouse  Pancreatic Islets and as a Regulator of RhoA and Rock1 Expresson in Mouse Organs” Rutgers University, 2002

Srilatha Raghuram       “Functional Analysis of the Ras-Activated Enhancer in the Osteopontin Promoter and Purification of RAE-Binding Proteins”            Rutgers University, 2002

Shashi Sharma, Tanupriya Contractor, Josephine Cassella – Interns from Kean College who earned a Masters degree in BioTechnology. 2003-2006

Dana Cifelli                 “Models for Investigating Functional Roles of Osteopontin: Characterization of anti-OPN Monoclonal Antibodies” Rutgers University, May 2009

Luke Coyle                 Osteopontin as a Regulator of Leukemia Inhibitory Factor mRNA levels in The Att-20 Mouse Pituitary Cell Line Rutgers University, Rutgers University, 2011

 

 

Ph.D. Theses Supervised:

David Dressler:            "A Rolling Circle Model for the Replication of φX DNA".  Harvard University, 1969.

Ann Baker Burgess:     AOn the  Proteins of Bacteriophage φX174".  Harvard University, 1969.

Makoto Iwaya:             "On the Mechanism of φX174 DNA Replication".  Harvard University, 1971.

Shlomo Eisenberg:       "Mechanism of φX174 RF Replication: Specific Initiation Sites for Synthesis of DNA".  McGill University, 1975.

Grant McFadden:         "Studies on the Replication of Bacteriophage φX174".  McGill University, 1978.

Christian Hours:           "Studies on DNA Replication in E. coli"  McGill University, 1978

Margaret Matthes:        "The Mechanism of Replication of φX174 DNA: Discontinuous Synthesis of the φX174 Viral Strand".  McGill University, 1980.

Louis Dubeau:             "Purification and Properties of the φX174 Gene A and A* Proteins".

McGill University, 1981.

Joseph Colasanti:         "Molecular and Genetic Analysis of the φX A* Gene and the             Escherichia coli Rep Gene".  University of Western Ontario, 1986.

Carol Gilchrist:            “Molecular Biological Studies on the rep Gene of Escherichia coli". University of Western Ontario, 1987.

Alison Connor:            “Regulation of MRP/PLF Gene Expression.” University of Western Ontario, 1989.

Ann Marie Craig:         “Analysis of Secreted Phosphoprotein (2ar; Osteopontin) Gene Expression and Association with Carcinogenesis”  University of Western Ontario, 1989.

Paul Waterhouse:         “Expression of Growth Factor Inducible Genes”  University of Western Ontario, 1989.

Shiaw-min Hwang:      “Regulation of Nitric Oxide Synthesis by Osteopontin in Primary Kidney Tubule Epithelial Cells”.  Rutgers University, 1993.

Xiaojia Guo:                “Identification of a Novel Ras-Activated Enhancer in the Osteopontin Promoter”.  Rutgers University, 1994.

Uriel Malyankar:          “Post-transcriptional Regulation of MRP/PLF is Altered during the Establishment of 3T3 Cell Lines from Primary Mouse Embryo Fibroblasts.”  Rutgers University, 1994

Ellen E. Rollo              “Inhibition of Nitric Oxide Production and Macrophage-mediated Cytotoxicity by Recombinant Human Osteopontin”.  Rutgers University, 1995.

Shyamala S. Rajan       “Purification and Characterization of the N-terminal Active Domain of the MouseTissue Inhibitor of Metalloproteinases-1 Expressed in Escherichia coli” Rutgers University, 1997

             Joseph F. Porter          “Characterization of the Metabolic Stimulation Caused by Tissue Inhibitor of Metalloproteinases-1 on Human Breast Cancer Cells” Rutgers University, 2004.

             Subarna A. Khan        “Regulation of CD44 and Apoptosis-Related Gene Bcl-XL Expression by Osteopontin. Rutgers University, 2004.

      Aaron J. Kowalski       “Creation, Characterization, and Application of Novel Anti-Osteopontin                                            Monoclonal Antibodies”. Rutgers University, 2004.

       Christian C. Kazanecki  “ Osteopontin and Cell Adhesion: Role of Post-translational                                                              Modifications and the C-terminal Region”  Rutgers University, 2007

Kathryn Wang             “Osteopontin: Role in Immune Regulation and Stress Responses”  Rutgers University, 2008

Sunanda Baliga                        “Acetaminophen Confers Neuroprotection during Early Cerebral                                                       Ischemia-Reperfusion” Rutgers University, 2009 (Joint supervision with                                           Professor Gary Merrill).

 

Kathryn Jaques            “Oxidant-induced injury in the mammalian myocardium: functional and molecular effects of acetaminophen”. Rutgers University  2010 (Joint supervision with  Professor Gary Merrill).

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Other Responsibilities and Activities:

Coorganizer with Dr. R. Miller (University of British Columbia) of the 1975 Canadian Biochemical Society Symposium on Nucleic Acid Structure and Function at Montebello, Quebec.  March 1975.

Organizer of the 1977 Cold Spring Harbor Meeting on "The Single-Stranded DNA Phages".  August, 1977.

Acting Chairman, Department of Biochemistry, McGill University, June-December 1977.

Visiting Professor Award from the Medical Research Council, 1978-79.  Sabbatical Research performed in the Imperial Cancer Research Fund Laboratories in London, England, with Dr. Lionel Crawford.

Member of the Scientific Board of the Center for Advanced Biotechnology and Medicine,   1988-1991.

Co-organizer with Dr. Renato Baserga of the meeting "Antisense Strategies"  sponsored by the New York Academy of Sciences Jan. 12-15, 1992, Philadelphia, PA.

Principal Organizer (with Drs. Bill Butler, Ann Chambers, and Don Senger) of the Meeting on "Osteopon­tin: Role in Cell Signalling and Adhesion". October 21-23, 1994.  Sponsored by the New York Academy of Sciences.  New Brunswick, New Jersey.

Co-organizer with Dr. William T. Butler of the Third International Conference on     Osteopontin and Related Proteins, San Antonio, Texas, May, 2002.


 

Postdoctoral Fellows: Present location, if known (circa 2000).

 

Loraine Larison, Harvard Univ., 1968-70 Location unknown

 

Ann C. Kato, McGill Univ. 1973-74  Currently Professor Adjoint, Dept. Physiology, Universite de Geneva, Switzerland.

 

Katalina Bartok, McGill University, 1974-75 Currently a Clinical Assistant Professor in the Department of Psychiatry, University of British Columbia, Vancouver, Canada

 

H. E. David Lane, McGill Univ., 1972-75, Professor, Centre de Recherche de biochem. et Genet. Cell. du CNRS, 118 route de Narbonne, 31062 Toulouse Cedex FRANCE

 

Seishi Takahashi, McGill Univ., 1975-1978, presently Chief Scientist at Mitsui Toatsu Chemicals, Chiba, Japan

 

Jacek Kowalski, McGill and Univ. Western Ontario, 1978-1988. Currently Principal Scientist for Wyeth-Lederle Vaccines in Pearl River New York.

 

Monica Närkhammar-Meuth, McGill Univ., 1978-79, in Sweden somewhere I believe, situation unknown.

 

Gregory P. Bondy, Univ. Western Ontario, 1981-2, University of British Columbia, Canada. Currently a full-time physician.

 

Hanna Bialkowska-Hobrzanska, Univ. Western Ontario, 1981-84, Director of the Molecular Biology Diagnostics Laboratory, St. Joseph's Health Care,London, Ontario.

 

Dylan R. Edwards, Univ. Western Ontario, 1981-1984. Currently Chair, Cancer Studies at the University of East Anglia, Norwich, U.K.

 

Craig L.J. Parfett, Univ. Western Ontario, 1983-1986, Staff Scientist, Mutagenesis Section, Environmental and Occupational Toxicology Division, Health Canada, Environmental Health Centre, 0803A, Tunney’s Pasture, Ottawa, ON, Canada K1A 0L2

 

James H. Smith, Univ. Western Ontario, 1982-1985, Currently Staff Scientist with Hamilton Thorne BioSciences, Beverly, MA.

 

Rama Khokha, UWO, 1985-1988 Assist. Prof., London Regional Cancer Center, London, Ontario

 

Rita Colella, Rutgers Univ., 1989-90, Currently Associate Professor, Department Anatomy & Neurobiology, Univ. Louisville, KY

 

Enzo T. Cocuzzi, Rutgers Univ. 1989-1992, Rutgers University. Currently Research Fellow, Pathology Department, Case Western Reserve University, Cleveland, Ohio

 

Bo Feng, Rutgers University, 1991-1994. Staff physician, OB/GYN, Robert Wood Johnson Medical School, UMDNJ, New Brunswick, NJ.

 

Cecilia A. Lopez (Cecilia A. Lopez-Chua) 1990-1994. Presently a patent specialist with Nixon Peabody LLP.

 

Shuba Krishna, 1998-2000. Currently back in India raising a family. Current position unknown.


 

Publications

 

1.         Denhardt, D.T. (1960).  A Possible Basis for Immunological Tolerance.  Nature 186:728-729.

 

2.         Denhardt, D.T., and Owen, R.D. (1960).  The Resistance of the Tolerant State to X-Irradiation.  Transplantation Bulletin 7:394-399.

 

3.         Denhardt, D.T., and Sinsheimer, R.L. (1965).  The Process of Infection with Bacteriophage φX174.  III. Phage Maturation and Lysis after Synchronized Infection.  J. Mol. Biol. 12:641-646.

 

4.         Denhardt, D.T., and Sinsheimer, R.L. (1965).  The Process of Infection with Bacteriophage φX174.  IV. Replication of the Viral DNA in a Synchronized Infection.  J. Mol. Biol. 12:647-662.

 

5.         Denhardt, D.T., and Sinsheimer, R.L. (1965).  The Process of Infection with Bacteriophage φX174.  V. Inactivation of the Phage-Bacterium Complex by Decay of 32P Incorporated in the Infecting Particle.  J. Mol. Biol. 12:663-673.

 

6.         Denhardt, D.T., and Sinsheimer, R.L. (1965).  The Process of Infection with Bacteriophage φX174.  VI.  Inactivation of Infected Complexes by Ultraviolet Irradiation. J. Mol. Biol. 12:674-694.

 

7.         Denhardt, D.T., and Silver, R.B. (1966).  An Analysis of the Clone Size Distribution of φX174 Mutants and Recombinants.  Virology 30:10-19.

 

8.         Denhardt, D.T. (1966).  A Membrane-Filter Technique for the Detection of Complementary DNA.  Biochem. Biophys. Res. Commun. 23:641-646.

 

9.         Denhardt, D.T., Dressler, D.H., and Hathaway, A. (1967).  The Abortive Replication of φX174 DNA in a Recombination-Deficient Mutant of Escherichia coli.  Proc. Natl. Acad. Sci. USA 57:813-820.

 

10.       Dressler, D.H., and Denhardt, D.T. (1968).  The Mechanism of Replication of φX174 Single-Stranded DNA.  Nature 219:346-351.

 

11.       Cairns, J., and Denhardt, D.T. (1968).  Effect of Cyanide and Carbon Monoxide on the Replication of Bacterial DNA in vivo.  J. Mol. Biol. 36:335-342.

 

12.       Steinberg, R.A., and Denhardt, D.T. (1968).  Inhibition of Synthesis of φX174 DNA in a Mutant Host Thermosensitive for DNA Synthesis.  J. Mol. Biol. 37:525-528.

 

13.       Denhardt, D.T., and Burgess, A.B. (1968).  DNA Replication in vitro.  I. Synthesis of Single-stranded φX174 DNA in Crude Lysates of φX-Infected E. coli.  Cold Spring Harbor Symp. Quant. Biol. 33:449-457.

 

14.       Denhardt, D.T. (1968).  DNA Replication in vitro.  II. Effect of Antiserum to E. coli DNA Polymerase on the Synthesis of φX174 DNA in Extracts of φX-Infected Cells.  Nature 220:131-134.

 

15.       Burgess, A.B., and Denhardt, D.T. (1969).  Studies on φX Proteins.  I. Phage Specific Proteins Synthesized after Infection of E. coli.  J. Mol. Biol. 45:377-386.

 

16.       Denhardt, D.T. (1969).  Formation of Ribosyl-Thymine in Escherichia coli: Studies on Pulse-Labeling with Thymine and Thymidine.  J. Biol. Chem. 244:2710-2715.

17.       Denhardt, D.T., and Marvin, D.A. (1969).  Altered Coding in Single-Stranded DNA Viruses.  Nature 221:769-770.

 

18.       Altman, S., and Denhardt, D.T. (1970).  Evidence for Preferential Breakage of the Minus Strand of φX174 Replicative Form DNA by a T4-Induced Endonuclease.  Biochim. Biophys. Acta 224:21-28.

 

19.       Iwaya, M., and Denhardt, D.T. (1971).  The Mechanism of Replication of φX174 Single-stranded DNA.  II. The Role of Viral Proteins.  J. Mol. Biol. 57:159-175.

 

20.       Schekman, R.W., Iwaya, M., Bromstrup, K., and Denhardt, D.T. (1971).  The Mechanism of Replication of φX174 Single-stranded DNA.  III. An Enzymatic Study of the Structure of the Replicative Form II DNA.  J. Mol. Biol. 57:177-199.

 

21.       Denhardt, D.T. (1972).  A Theory of DNA Replication.  J. Theoretical Biol. 34:487-508.

 

22.       Denhardt, D.T., Iwaya, M., and Larison, L. (1972).  The rep Mutation.  II. Its Effect on Escherichia coli and on the Replication of Bacteriophage φX174.  Virology 49:486-496.

 

23.       Iwaya, M., and Denhardt, D.T. (1973).  The Mechanism of Replication of φX174 Single-stranded DNA.  IV. The Parental Viral Strand is not Conserved in the Replicating DNA Structure.  J. Mol. Biol. 73:279-290.

 

24.       Iwaya, M., and Denhardt, D.T (1973).  The Mechanism of Replication of φX174 Single-stranded DNA.  V. Dispersive and Conservative Transfer of Parental DNA into Progeny DNA.  J. Mol. Biol. 73:291-305.

 

25.       Kato, A.C., Bartok, K., Fraser, M.J., and Denhardt, D.T. (1973).  Sensitivity of Superhelical DNA to a Single-Strand Specific Endonuclease.  Biochim.  Biophys. Acta 308:68-78.

 

26.       Denhardt, D.T., and Kato, A.C. (1973).  Comparison of the Effect of Ultraviolet Radiation and Ethidium Bromide Intercalation on the Conformation of Superhelical φX174 Replicative Form DNA.  J. Mol. Biol. 77:479-494.

 

27.       Denhardt, D.T., Iwaya, M., McFadden, G., and Schochetman, G. (1973).  The Mechanism of Replication of φX174 Single-Stranded DNA.  VI. Requirement for Ribonucleoside Triphosphates and DNA Polymerase III.  Can. J. Biochem. 51:1588-1597.

 

28.       Iwaya, M., Eisenberg, S., Bartok, K., and Denhardt, D.T. (1973).  Mechanism of Replication of Single-Stranded φX174 DNA.  VII. Circularization of the Progeny Viral Strand.  J. Virol. 12:808-818.

 

29.       Eisenberg, S., and Denhardt, D.T. (1974).  Structure of Nascent φX174 Replicative Form: Evidence for Discontinuous DNA Replication.  Proc. Natl. Acad. Sci. USA. 71:984-988.

 

30.       Kato, A.C., Borstad, L., Fraser, M.J., and Denhardt, D.T. (1974).  Isolation of Repeated and Self-Complementary Sequences from E. coli DNA.  Nucl. Acids Res. 1:1539-1548.

 

31.       Lane, H.E.D., and Denhardt, D.T. (1974).  The rep Mutation.  III. Altered Structure of the Replicating Escherichia coli Chromosome.  J. Bacteriol. 120:805-814.

 

32.       McFadden, G., and Denhardt, D.T. (1974).  The Mechanism of Replication of φX174 Single-stranded DNA.  IX. Requirement for the E. coli dnaG Protein.  J. Virol. 14:1070-1075.

33.       Eisenberg, S., and Denhardt, D.T. (1974).  The Mechanism of Replication of φX174 Replicative Form DNA.  X. Distribution of the Gaps in Nascent φX174 RF.  Biochem. Biophys. Res. Comm. 61:532-537.

 

34.       Lane, H.E.D., and Denhardt, D.T. (1975).  The rep Mutation.  IV. Slower Movement of Replication Forks in Escherichia coli rep Strains.  J. Mol. Biol. 97:99-112.

 

35.       Bartok, K., Harbers, B., and Denhardt, D.T. (1975).  Isolation and Characterization of Self-Complementary Sequences from φX174 Viral DNA.  J. Mol. Biol. 99:93-105.

 

36.       Carter, B.J., Khoury, G., and Denhardt, D.T. (1975).  Physical Map and Strand Polarity of Specific Fragments of Adenovirus-Associated Virus DNA Produced by Endonuclease R.EcoRI.  J. Virol. 16:559-568.

 

37.       Denhardt, D.T., Eisenberg, S., Harbers, B., Lane, H.E. David, and McFadden, G. (1975).  Replication of φX174 in Escherichia coli: Structure of the Replicating Intermediate and the Effect of Mutations in the Host lig and rep Genes.  In DNA Synthesis and Its Regulation.  ICN-UCLA Conference.  Edited by M. Goulian and P. Hanawalt.  W.A. Benjamin & Co. 3, 398-422.

 

38.       Eisenberg, S., Harbers, B., Hours, C., and Denhardt, D.T. (1975).  The Mechanism of Replication of φX174 DNA.  XII.  Non-random Location of Gaps in Nascent φX174 RF II DNA.  J. Mol. Biol. 99:107-123.

 

39.       McFadden, G., and Denhardt, D.T. (1975).  The Mechanism of Replication of φX174 DNA.  XIII. Discontinuous Synthesis of the Complementary Strand in an Escherichia coli Host with a Temperature-Sensitive Polynucleotide Ligase.  J. Mol. Biol. 99:125-142.

 

40.       Denhardt, D.T. (1975).  The Single-stranded DNA Bacteriophages.  Crit. Reviews Microbiology 4:161-223.

 

41.       Bartok, K., and Denhardt, D.T. (1976).  Site of Cleavage of Superhelical φX174 Replicative Form DNA by the Single-strand Specific Neurospora crassa Nuclease.  J. Biol. Chem. 251:530-535.

 

42.       Denhardt, D.T., Eisenberg, S., Bartok, K., and Carter, B. (1976).  Multiple Structures of Adeno-Associated Virus DNA: Analysis of Terminally Labeled Molecules with Endonuclease R.Hae III.  J. Virol. 18:672-684.

 

43.       McFadden, G., and Denhardt, D.T. (1977).  Contrasting Effects of 2'3' Dideoxyadenosine on Two Steps in the Replication of φX174 DNA in Escherichia coli.  Virology 76:870-875.

 

44.       Denhardt, D.T. (1977).  The Isometric Single-stranded DNA Phages.  Chapter I in Comprehensive Virology, Vol. 7.  Edited by H. Fraenkel-Conrat and R.R. Wagner.  Plenum Press, New York.  pp. 1-104.

 

45.       Denhardt, D.T. (1977).  The Transmission of the Genetic Message: DNA Structure and Function.  In Comprehensive Biochemistry, Vol. 24, Chapter I.  pp 1-53.  Biological Information Transfer.  A. Neuberger (Ed.) Elsevier/North Holland Biomedical Press.

 

46.       Woodworth-Gutai, M., Lebowitz, J., Kato, A.C., and Denhardt, D.T. (1977).  Ultraviolet light irradiation of PM2 superhelical DNA.  Nucl. Acids Res. 4:1243-1256.

 

47.       Miyamoto, C., and Denhardt, D.T. (1977).  Evidence for the Presence of Ribonucleotides at the 5' Termini of Some DNA Molecules Isolated from Escherichia coli polAex2.  J. Mol. Biol. 116:681-707.

 

48.       Takahashi, S., Hours, C., and Denhardt, D.T. (1977).  Specialized Transduction of the rep Gene of E. coli. FEMS Letters 2:279-283.

 

49.       Denhardt, D.T., and Hours, C. (1978).  The Present Status φX174 DNA Replication In Vivo.  In DNA Synthesis: Present and Future, Edited by I. Molineux and M. Kohiyama, Plenum Press, pp. 693-704.

 

50.       Denhardt, D.T. (1978).  Evidence for the Absence of Triphosphate Termini from the 5' Ends of Newly Synthesized E. coli DNA Molecules.  In DNA Synthesis: Present and Future, Edited by I. Molineux and M. Kohiyama, Plenum Press, pp. 321-332.

 

51.       Matthes, M., and Denhardt, D.T. (1978).  Comparison of Restriction Endonuclease HaeIII Fragments of φX174, φXtb, and St-1: Evidence that φXtb is ST-1.  Virology 85:626-627.

 

52.       Hours, C., Matthes, M., Miyamoto, N., and Denhardt, D.T. (1978).  Nascent Pieces of φX174 Viral Strand DNA are Shorter Than Unit-Length and Derived from All Regions of the Genome.  In The Single-stranded DNA Phages.  Edited by D.T. Denhardt, D.H. Dressler, and D.S. Ray.  Cold Spring Harbor Monograph.  pp. 245-253.

 

53.       Takahashi, S., Hours, C., Iwaya, M., Lane, H.E. David, and Denhardt, D.T. (1978).  The Escherichia coli rep Gene.  In The Single-Stranded DNA Phages.  Edited by D.T. Denhardt, D.H. Dressler and D.S. Ray.  Cold Spring Harbor Monograph.  pp. 393-400.

 

54.       Denhardt, D.T. (1978).  Overview: A Comparison of the Isometric and Filamentous Phages.  In The Single-Stranded DNA Phages.  Edited by D.T. Denhardt, D.H. Dressler, and D.S. Ray.  Cold Spring Harbor Monograph.  pp. 645-655.

 

55.       Kowalski, J., and Denhardt, D.T. (1978).  Most Short DNA Molecules Isolated from 3T3 Cells are Not Nascent.  Nucl. Acids Res. 5:4355-4373.

 

56.       Hours, C., and Denhardt, D.T. (1979).  "Nick Translation" in Escherichia coli rep strains deficient in DNA Polymerase I Activities.  Molec. Gen. Genetics 172:73-80.

 

57.       Denhardt, D.T., Kowalski, J., and Miyamoto, C. (1979).  Evidence for Many Non-nascent Molecules the Size of Okazaki Fragments in Escherichia coli and 3T3 cells: Very Few Have 5' Terminal Ribonucleo­tides.  Cold Spring Harbor Symp. Quant. Biol. 43:243-251.

 

58.       Takahashi, S., Hours, C., Chu, A., and Denhardt, D.T. (1979).  The rep mutation VI. Purification and Properties of the Escherichia coli rep Protein - DNA Helicase III.  Can. J. Biochem. 57:855-866.

 

59.       Denhardt, D.T. (1979).  DNA Gyrase and DNA Unwinding.  Nature - News and Views 280:196-198.

 

60.       Kowalski, J., and Denhardt, D.T. (1979).  Ribonucleotides in DNA Newly Synthesized in 3T6 Cells In Vivo.  Nature 283:704-706.

 

61.       Matthes, M., and Denhardt, D.T. (1980).  The Mechanism of Replication of φX174 DNA  XVI. Evidence that the φX Viral Strand is Synthesized Discontinuously.  J. Mol. Biol. 136:45-63.

 

62.       Crawford, L.V., Lane, D.P., Denhardt, D.T., Harlow, E.E., Nicklin, P.M., Osborn, K., and Pim, D.C. (1980).  Characterization of the Complex between SV40 Large-T Antigen and the 53K Host Protein in Transformed Mouse Cells.  Cold Spring Harbor Symposium on Quantitative Biology 44:179-187.

 

63.       Denhardt, D.T., and Crawford, L.V. (1980).  Simian Virus 40 T-antigen: Identification of Tryptic Peptides in the C-terminal Region and Definition of the Reading Frame.  J. Virol. 34:315-329.

 

64.       Dubeau, L., Hours, C., and Denhardt, D.T. (1981).  The Mechanism of Replication of φX174  XVII. Purification and Partial Characterization of the Gene A and A* Proteins.  Can. J. Biochem. 59:106-115.

 

65.       Dubeau, L., and Denhardt, D.T. (1981).  The Mechanism of Replication of φX174.  XVIII. Gene A and A* Proteins of φX174 Bind Tightly to φX174 Replicative Form DNA.  Biochim. Biophys. Acta 653:52-60.

 

66.       Närkhammar-Meuth, M., Kowalski, J., and Denhardt, D.T. (1981).  Both Strands of Polyoma DNA are Replicated Discontinuously with RNA Primers in vivo.  J. Virol. 39:21-30.

 

67.       Matthes, M., Weisbeek, P.J., and Denhardt, D.T. (1982).  The Mechanism of Replication of φX174  XIX. Initiation of φX174 Viral Strand DNA Synthesis at Internal Sites on the Genome.  J. Virol. 42:301-305.

 

68.       Matthes, M., and Denhardt, D.T. (1982).  The Mechanism of Replication of φX174  XX. Studies on the Sensitivity of Nascent DNA to Single-strand Specific Nucleases.  J. Virol. 42:12-19.

 

69.       Kowalski, J., and Denhardt, D.T. (1982).  Adenovirus DNA Replication in vivo: Properties of Short DNA Molecules Extracted from Infected Cells.  Biochim. Biophys. Acta 698:260-270.

 

70.       Denhardt, D.T. (1982) This Week's Citation Classic (Publication #8) Current Contents 25 (#43), 22.

 

71.       Denhardt, D.T., and Miyamoto, C. (1983).  Characteristics of the Nascent and Non-nascent Small DNA Molecules Found in Escherichia coli.  J. Mol. Biol. 165:419-442.

 

72.       Bondy, G.P., and Denhardt, D.T. (1983) Exposure of JB-6 Mouse Epidermal Cells to 12-O-Tetradecanoylphorbol-13-acetate is not Accompanied by a Significant Change in Total DNA-Cytosine Methylation.  Carcinogenesis 4:1599-1603.

 

73.       Denhardt, D.T. (1983) Replication of DNA.  Monograph #120 in the Oxford/Carolina Biology Readers Series, Edited by J.J. Head.  Carolina Biological Supply Company, Burlington, North Carolina.

 

74.       Bialkowska-Hobrzanska, H., and Denhardt, D.T. (1984) The rep Mutation  VII. Cloning and Analysis of the Functional rep gene of Escherichia coli K-12.  Gene 28:93-102.

 

75.       Denhardt, D.T. (1984) DNA Replication: A Twofold Amplified View.  Nature News and Views 309:515.

 

76.       Edwards, D.R., and Denhardt, D.T. (1984) Regulation of Nuclear and Mitochondrial RNA Production After Serum Stimulation of Quiescent Mouse Cells.  In the Proceedings of the Tenth International Cell Cycle Conference:  Growth, Cancer and the Cell, Cycle, ed. P. Skehan and S.J. Friedman, The Humana Press, Clifton, N.J. pp. 117-124.

 

77.       Edwards, D.R., and Denhardt, D.T. (1985) A Study of Mitochondrial and Nuclear Transcription with Cloned cDNA Probes: Changes in the Relative Abundance of Mitochondrial Transcripts After Stimulation of Quiescent Mouse Fibroblasts.  Exp. Cell Res. 157:127-143.

 

78.       Colasanti, J., and Denhardt, D.T. (1985) Expression of the Cloned Bacteriophage φX174 A* Gene in Escherichia coli Inhibits DNA Replication and Cell Division.  J. Virol. 53:807-813.

 

79.       Kowalski, J., Smith, J.H., Ng, N., and Denhardt, D.T. (1985) Vectors for the Direct Selection of cDNA Clones Corresponding to Mammalian Cell mRNA of Low Abundance.  Gene 35:45-54.

 

80.       Denhardt, D.T., and Faust, E.A. (1985) Eukaryotic DNA Replication.  BioEssays 2:148-154.

 

81.       Bialkowska-Hobrzanska, H., Gilchrist, C.A., and Denhardt, D.T. (1985) The Escherichia coli rep gene: Identification of the promoter and N-terminus of the rep protein.  J. Bact. 164:1004-1010.

 

82.       Edwards, D.R., Parfett, C.L.J., and Denhardt, D.T. (1985) A pBR322-derived Vector for Cloning Blunt-ended cDNA: Its Use to Detect Molecular Clones of Low Abundance mRNAs.  DNA 4:401-408.

 

83.       Edwards, D.R., Parfett, C.L.J., and Denhardt, D.T. (1985) Transcriptional Regulation of Two Serum-Induced RNAs in Mouse Fibroblasts: Equivalence of One Species to B2 Repetitive Elements.  Mol. Cell. Biol. 5:3280-3288.

 

84.       Parfett, C.L.J., Hamilton, R.T., Howell, B., Edwards, D.R., Nilsen-Hamilton, M., and Denhardt, D.T. (1985) Characterization of a cDNA Clone Encoding Murine Mitogen-Regulated Protein: Regulation of mRNA Levels in Mortal and Immortal Cell Lines.  Mol. Cell. Biol. 5:3289-3292.

 

85.       Denhardt, D.T. (1985) Stacking Polyacrylamide Baby Gels.  Focus 7 (3), 17.

 

86.       Denhardt, D.T., Hamilton, R.T., Parfett, C.L.J., Edwards, D.R., St. Pierre, R., Waterhouse, P., and Nilsen-Hamilton, M. (1986) Close Relationship of the Major Excreted Protein of Transformed Murine Fibroblasts to Thiol-dependent Cathepsins.  Cancer Res. 46:4590-4593.

 

87.       Edwards, D.R., Waterhouse P., Holman, M.L., and Denhardt, D.T. (1986) A Growth-responsive Gene (16C8) in Normal Mouse Fibroblasts Homologous to a Human Collagenase Inhibitor with Erythroid-potentiating Activity: Evidence for Inducible and Constitutive Transcripts.  Nucleic Acids Res. 14:8863-8878.

 

88.       Denhardt, D.T., Edwards, D.R., and Parfett, C.L.J. (1986) Gene Expression During the Mammalian Cell Cycle.  Biochim. Biophys. Acta 865:83-125.

 

89.       Gilchrist, C.A., and Denhardt, D.T. (1987) Escherichia coli rep gene: Sequence of the gene, the encoded helicase, and its homology with uvrD.  Nucl. Acids Res. 15:465-475.

 

90.       Smith, J.H., and Denhardt, D.T. (1987) Molecular Cloning of a Tumor Promotor-Inducible mRNA Found in JB6 Mouse Epidermal Cells: Induction is Stable at High but not at Low Densities.  J. Cell. Biochem. 34:13-22.

 

91.       Denhardt, D.T., Edwards, D.R., Parfett, C.L.J., and Smith, J. (1987) Regulation of Gene Expression in Mortal and Immortal Murine Fibroblasts - 3T3 but not 3T12 Cells Make MRP/Proliferin.  In Molecular Mechanisms in the Regulation of Cell Behaviour, pp59-64. Ed. by C. Waymouth, Alan R. Liss, Inc., N.Y.

 

92.       Denhardt, D.T., and Colasanti, J. (1987) A Survey of Vectors for Regulating Expression of Cloned DNA in E. coli.  Chapter 9, pp. 179-203.  In Vectors: A Survey of Molecular Cloning Vectors and Their Uses, Edited by R. Rodriguez and D.T. Denhardt.  Butterworth Publishers, Stoneham, Mass.

 

93.       Denhardt, D.T., Edwards, D.R., Kowalski, J., Parfett, C.L.J., and Waterhouse, P. (1987) Specialized Plasmid Vectors for Cloning cDNA.  Chapter 12, pp237-251.  In Vectors: A Survey of Molecular Cloning Vectors and Their Uses, Edited by R. Rodriguez and D.T. Denhardt.  Butterworth Publishers, Stoneham, Mass.

 

94.       Colasanti, J., and Denhardt, D.T. (1987) Mechanism of Replication of Bacteriophage φX174  XXII Site-Specific Mutagenesis of the A* Gene Reveals that A* Protein is not Essential for φX174 DNA Replication.  J. Mol. Biol. 197:47-54.

 

95.       Colasanti, J., and Denhardt, D.T. (1987) The Escherichia coli rep Mutation X: Consequences of Increased and Decreased Rep Protein Levels.  Mol. Gen. Genet. 209:382-390.

 

96.       Khokha, R., and Denhardt, D.T. (1987) On the Use of Anti-sense RNA: Down-regulation of mRNA Encoding a Metalloproteinase Inhibitor.  Anticancer Res. 7:653-660.

 

97.       Denhardt, D.T., Greenberg, A.H., Egan, S.E., Hamilton, R., and Wright, J.A. (1987) Cysteine Protease Cathepsin L Expression Correlates with the Metastatic Potential of H-ras-transformed Murine Fibroblasts.  Oncogene 2:55-59.

 

98.       Edwards, D.R., Parfett, C.L.J., Smith, J.H., and Denhardt, D.T. (1987) Evidence that Post-transcriptional Changes in the Expression of Mitogen Regulated Protein Accompany Immortalization of Mouse Cells.  Biochem. Biophys. Res. Commun. 147:467-473.

 

99.       Denhardt, D.T., and Kowalski, J. (1988) Is There Induced DNA Repair in Mammalian Cells? Bioessays 9:70-72.

 

100.     Craig, A.M., Nemir, M., Mukherjee, B.B., Chambers, A.F., and Denhardt, D.T. (1988) Identification of the Major Phosphoprotein Secreted by Many Rodent Cell Lines as 2ar/osteopontin: Enhanced Expression inn H-ras-transformed 3T3 cells.  Biochem. Biophys. Res. Comm. 157:166-173.

 

101.     Denhardt, D.T., Craig, A.M., and Smith, J.H. (1989) Regulation of Gene Expression by the Tumor Promoter TPA.  In Genes and Signal Transduction in Multistage Carcinogenesis, Edited by N.H. Colburn. Marcel Dekker Publishers (pp. 167-189).

 

102.     Parfett, C.L.J., and Denhardt, D.T. (1989) Changes in Gene Expression During Senescence/Immortaliza­tion of Mouse Embryo Fibroblasts.  In Growth Control During Cell Aging, Edited by H.R. Warner and E. Wang.  pp 31-40  CRC Press.

 

103.     Smith, J.H., and Denhardt, D.T. (1989) Effects of Non-Phorbol Tumor Promoters and Tumor Promotion Inhibitors on a TPA-inducible Gene ("2ar") Found in Mouse JB6 Epidermal Cells: Evidence for Two Mechanisms of Induction J. Cell. Physiol. 139:189-195.

 

104.     Yagel, S., Warner, A.H., Nellans, H.N., Lala, P.K., Waghorne, C., and Denhardt, D.T. (1989) Suppression by Cathepsin L Inhibitors of the Invasion of Amnion Membranes by Murine Cancer Cells.  Cancer Res. 49:3553-3557.

 

105.     Khokha, R., Waterhouse, P., Yagel, S., Lala, P.K., Norton, G., and Denhardt, D.T. (1989) Antisense RNA-Induced Reduction in Murine TIMP Levels Confers Oncogenicity on Swiss 3T3 Cells.  Science 243:947-950.

 

106.     Yagel, S., Khokha, R., Denhardt, D.T., Kerbel, R.S., Parhar, R.S., and Lala, P.K. (1989) Mechanisms of Cellular Invasiveness: A Comparison of Amnion Invasion In Vitro and Metastatic Behaviour In Vivo.  J. Natl. Cancer Inst. 81:768-775.

 

107.     Kowalski, J., and Denhardt, D.T. (1989) Regulation of the mRNA for Monocyte-Derived Neutrophil-Activating Peptide in Differentiating HL60 Promyelocytes.  Molec. Cell. Biol. 9:1946-1957.

 

108.     Parfett, C.L.J., Hofbauer, R., Brudzynski, K., Edwards, D.R., and Denhardt, D.T. (1989) Differential screening of a cDNA library with cDNA probes amplified in a heterologous host: Isolation of murine GRP78 (BiP) and other serum-regulated low-abundance mRNAs.  Gene 82:291-303.

 

109.     Craig, A.M., Smith, J.H., and Denhardt, D.T. (1989) Osteopontin, a Transformation-Associated Cell Adhesion Phosphoprotein, is Induced by 12-0-Tetradecanoylphorbol-13-Acetate in Mouse Epidermis.  J. Biol. Chem. 264: 9682-9689.

 

110.     Connor, A.M., Waterhouse, P., Khokha, R., and Denhardt, D.T. (1989) Characterization of a Mouse Mitogen Regulated Protein/Proliferin Gene and its Promoter:  A Member of the Growth Hor­mone/Prolactin Gene Superfamily.  Biochim. Biophys. Acta 1009:75-82

 

111.     Khokha, R., and Denhardt, D.T. (1989) Matrix metalloproteinases and tissue inhibitor of metallopro­teinases (TIMP):  A review of their role in tumorigenesis and tissue invasion. Invasion and Metastasis  9: 391-405.

 

112.     Chambers, A.F., and Denhardt, D.T. (1990) Abatement of Gene Expression Using Antisense Oligodeoxynucleo­tides. Pharmaceutical Technology 14:24-28.

 

113.     Waterhouse, P., Khokha, R., and Denhardt, D.T. (1990) Modulation of Translation by the 5' Leader Sequence of the mRNA Encoding Murine Tissue Inhibitor of Metalloproteinases. J. Biol. Chem. 265:5585-5589.

 

114.     Craig, A.M., Bowden, G.T., Chambers, A.F., Spearman, M., Greenberg, A.H., Wright, J., McLeod, M., and Denhardt, D.T. (1990) Secreted Phosphoprotein mRNA is Induced During Multistage Carcinogenesis in Mouse Skin and Correlates with the Metastatic Potential of Murine Fibroblasts.  Int. J. Cancer 46:133-137.

 

115.     Guo, X., Chambers, A.F., Parfett, C.L.J., Waterhouse, P., Murphy, L.C., Reid, R., Craig, A.M., Edwards, D.R., and Denhardt, D.T. (1990) Identification of a Serum-inducible mRNA (5B10) as the Mouse Homolog of Calcyclin:  Tissue Distribution and Expression in Metastatic, Ras-transformed NIH 3T3 Cells.  Cell Growth and Differentiation 1:333-338.

 

116.     Noda, M., Vogel, R.L., Craig, A.M., Prahl, J., DeLuca, H.F., and Denhardt, D.T. (1990) Identification of a DNA Sequence Responsible for Binding of the 1,25-dihydroxyvitamin D3 Receptor and 1,25-dihydroxyvi­tamin D3 Enhancement of Mouse Secreted Phosphoprotein 1 (Spp-1, or osteopontin) Gene Expression.  Proc. Natl. Acad. Sci. USA 87:9995-9999.

 

117.     Chan, J.C., Scanlon, M., Denhardt, D.T., Singh, K., Mukherhee, B.B., Farach-Carson, M.C., and Butler, W.T. (1990) Immunochemical Comparison of Transformation Associated Protein and Secreted Phosphopro­tein. Int. J. Cancer 46: 864-870.

 

118.     Somerman, M.J., Shroff, B., Agraves, W.S., Morrison, G., Craig, A.M., Denhardt, D.T., Foster, R.A., and Sauk, J.J. (1990) Expression of Attachment Proteins during Cementogenesis. J. Biol. Buccale 18:207‑214.

 

119.     Denhardt, D.T., Edwards, D.R., McLeod, M., Norton, G., Parfett, C.L.J., and Zimmer, M. (1991) Spontaneous Immortalization of Mouse Embryo Cells: Strain Differences and Changes in Gene Expression with Particular Reference to Retroviral gag-pol Genes.  Experimental Cell Res. 192:128-136.

 

120.     Craig, A.M. and Denhardt, D.T. (1991) The Murine Gene Encoding Secreted Phosphoprotein (Osteopon­tin):  Promoter Structure, Activity and Induction in vivo by Estrogen and Progesterone. Gene 100:163‑171.

 

121.     Khokha, R., Waterhouse, P., Lala, P., Zimmer, M., and Denhardt, D.T. (1991) Increased Proteinase Expression during Tumor Progression of Cell Lines Down-modulated for TIMP Levels: A New Transformation Paradigm? J. Cancer Res. Clin. Oncology 117:333-338.

 

122.     Hofbauer, R.,and Denhardt, D.T. (1991) Cell Cycle-regulated and Proliferation Stimulus-responsive Genes. Crit. Rev. Eukaryotic Gene Exp. 1:247-300.

 

123.     Nilsen-Hamilton, M., Jang, Y.-J., Delgado, M., Shim, J.-K., Bruns, K., Chiang, C.-P., Fang, Y., Parfett, C.L.J., Denhardt, D.T., and Hamilton, R.T. (1991) Regulation of the Expression of Mitogen-regulated Protein (MRP; proliferin) and Cathepsin L in Cultured Cells and in the Murine Placenta. Molec. Cell. Endocrin. 77:115-122. 

 

124.     Hamilton, R.T., Bruns, K.A., Delgado, M.A., Shim, J.-K., Fang, Y., Denhardt, D.T., and Nilsen-Hamilton, M. (1991) Developmental Expression of Cathepsin L and c-rasHa in the Mouse Placenta. Molec.Reprod. Develop.30:285-292.

 

125.     Denhardt, D.T., Khokha, R.K., Yagel, S., Overall, C.M., and Parhar, R.S. (1992) Oncogenic Consequences of Down-modulating TIMP Expression in 3T3 Cells with Antisense RNA.  In "Matrix Metallopro­teinases and Inhibitors" Edited by H. Birkedal-Hansen, Z. Werb, H. Welgus and H. van Wart.  Academic Press, NY. MATRIX, Supplement No. 1, pp281-285.

 

126.     Rittling, S.R., and Denhardt, D.T. (1992) p53 Mutations in Spontaneously Immortalized 3T12 but not 3T3 Mouse Embryo Cells. Oncogene 7:935-942.

 

127.     Chambers, A.F., Behrend, E.I., Wilson, S.M., and Denhardt, D.T. (1992) Induction of Expression of Osteopontin (OPN; Secreted Phosphoprotein) in Metastatic, Ras-transformed NIH3T3 cells.  Anticancer Res. 12:43-48.

 

128.     Waterhouse, P., Parhar, R. S., Guo, X., Lala, P. K., and Denhardt, D.T. (1992) Regulated Temporal and Spatial Expression of the Calcium-binding Proteins Calcyclin and OPN (osteopontin) in Mouse Tissues during Pregnancy.  Molec. Reprod. Develop. 32:315-323.

 

129.     Chambers, A.F., Colella, R., Denhardt, D.T., and Wilson, S.M. (1992) Increased Expression of Cathep­sins L and B, and Decreased Activity of their Inhibitors, in Metastatic, ras-transformed NIH 3T3 cells. Molec. Carcinogenesis 5:238-245.

 

130.     Denhardt, D.T.  (1992)  Antisense Strategies Come of Age.  The New Biologist 4:473-481.

 

131.     Denhardt, D.T. (1992) Mechanism of Action of Antisense RNA:  Sometime Inhibition of Transcription, Processing, Transport or Translation.  In Antisense Strategies.  Edited by R. Baserga and D.T. Denhardt.  Published by the  New York Academy of Sciences Vol. 660:70-76.

 

132.     Feng, B. and Denhardt, D.T. (1992) Inhibition of Processing of the Primary Transcript of the Gene Encoding Tissue Inhibitor of Metalloproteinases (TIMP) by Antisense TIMP RNA in Mouse 3T3 Cells.  In Antisense Strategies, published by the New York Academy of Sciences Vol. 660:280-282.

 

Cocuzzi, E.T., Walther, S.E., Rajan, S., and Denhardt, D.T. (1992) Expression and Purification of Mouse TIMP-1 from E. coli.  FEBS Letters, 304:375-378.

 

134.     Colella, R.,Chambers, A.F., and Denhardt, D.T. (1993) Anticarcinogenic Activities of Naturally Occurring Cysteine Proteinase Inhibitors. In "Proteinase Inhibitors as Cancer Chemopreventive Agents" Edited by W. Troll and A. Kennedy.  Plenum Publishing Corp. pp 199-216.

 

135.     Behrend, E.I., Chambers, A.F., Wilson, S.M., and Denhardt, D.T.  (1993) Comparative analysis of two alternative first exons reported for the mouse osteopontin gene.  J. Biol. Chem. 268:11172-11175.

 

136.     Lopez, C.A., Hoyer, J.R., Wilson, P.D., Waterhouse, P., and Denhardt, D.T.  (1993)  Heterogeneity of osteopontin expression among nephrons in mouse kidneys.  Laboratory Investigation 69:355-363.

 

137.     Krook, A., Rapoport, M.J., Anderson, S., Pross, H., Zhou, Y.C., Denhardt, D.T., Delovitch, T.L., and Haliotis, T. (1993) p21ras and protein kinase C function in distinct and interdependent signaling pathways in C3H 10T2 fibroblasts. Mol. Cell. Biol. 13:1471-1479.

 

138.     Waterhouse, P., Denhardt, D.T., and Khokha, R. (1993) Temporal expression of tissue inhibitors of metalloproteinases in mouse reproductive tissues during gestation. Molec. Reprod. Develop. 35:219-226.

 

139.     Denhardt, D.T., Feng, B., Edwards, D.R., Cocuzzi, E.T., and Malyankar, U.M. (1993) Tissue inhibitor of metalloproteinases (TIMP, aka EPA):  Structure, control of expression and biological functions.  Pharmacology and Therapeutics 59:329-341.

 

140.     Hofbauer, R., and Denhardt, D.T. (1993) Isolation of cDNA clones of low-abundance mRNAs:  Alternative strategies in differential and subtractive hybridization. In Methods in Molecular Genetics 2:3-25.  Edited by  K. Adolph, Academic Press, Inc.

 

141.     Denhardt, D.T., and Guo, X. (1993) Osteopontin: a protein with diverse functions.  FASEB J. 7:1475-1482.

 

142.     Denhardt, D.T. (1994) The Isometric Phages. In The Encyclopedia of Virology  Edited by R.G. Webster and A. Granoff.  Academic Press Inc.  pp 989-996.

 

 143.    Cocuzzi, E.T., Walther, S.E., and Denhardt, D.T. (1994) Expression and secretion of active mouse TIMP-1 using a Baculovirus expression vector. Inflammation 18:35-43

 

144.     Hwang, S.-m., Lopez, C.A., Heck, D.E., Gardner, C.R., Laskin, D.L., Laskin, J.D., and Denhardt, D.T. (1994) Osteopontin inhibits induction of nitric oxide synthase gene expression by inflammatory mediators in mouse kidney epithelial cells. J. Biol. Chem. 269:711-715.

 

145.     Giachelli, C., Bae, N., Almeida, M., Denhardt, D.T. Alpers, C.E., and Schwartz, S. (1994) Osteo­pontin is elevated during neointimal formation in rat arteries and is a novel component of human atherosclerotic plaques. J. Clin. Invest. 92:1686-1696.

 

146.     Giachelli, C.M., Pichler, R., Lombardi, D., Denhardt, D.T., Alpers, C., Schwartz, S., and Johnson, R.J. (1994) Osteopontin expression in angiotensin II-induced tubulointerstitial nephritis. Kidney Int. 45:515-524.

 

147.     Malyankar, U.M., Rittling, S.R., Connor, A., and Denhardt, D.T. (1994) The MRP/proliferin transcript is degraded in primary mouse embryo fibroblast but not 3T3 nuclei:  Altered RNA processing correlates with immortalization. Proc. Natl. Acad. Sci. USA  91:335-339.

 

148.     Behrend, E.I., Craig, A.M., Wilson, S.M., Denhardt, D.T., and Chambers, A.F. (1994) Reduced malignancy of ras-transformed NIH3T3 cells expressing antisense osteopontin RNA.  Cancer Res. 54:832-837.

 

149.     Xuan, J.-W., Kowalski, J., Chambers, A.F., and Denhardt, D.T. (1994) A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase. Genomics 20:129-131.

 

150 .    Hwang, S.-m.,  Wilson, P.D., Laskin, J., and Denhardt, D.T. (1994) Age-related changes in osteopontin and nitric oxide synthase mRNA levels in human kidney proximal tubule epithelial cells:  Response to hypoxia and reoxygen­ation. J. Gen. Physiol. 160:61-68.

 

151.     Denhardt, D.T. and Chambers, A.F. (1994) Overcoming obstacles to metastasis - defense against host defenses:  Osteopontin (OPN) as a shield against attack by cytotoxic host cells.  J. Cellular Biochem.  56:48-51.

 

152.     Garcia-Welsh, A., Laskin, D.L., Hwang, S.-m., Denhardt, D.T., and Laskin, J.D. (1994) Production of nitric oxide by differentiated LSTRA cells is associated with expression of macrophage-inducible nitric oxide synthase.  J. Leukocyte Biol. 56:488-494.

 

153.     Denhardt, D.T., Rajan, S., and Walther, S.E. (1994) Structure-function studies of mouse tissue inhibitor of metalloproteinase-1.  In INHIBITION OF MATRIX METALLOPROTEINASES: THERAPEUTIC POTENTIAL.  Annals of the NY Academy of Sciences 732:65-74.

 

154.     Guo, X., Zhang, Y.P., Mitchell, D.A., Denhardt, D.T. and Chambers, A.F.  (1995)  Identification of a ras-activated enhancer in the mouse osteopontin gene and its interaction with a putative ETS-related transcription factor whose activity correlates with the metastatic potential of the cell.  Mol. Cell. Biol. 15:476-487.

 

155.     Lopez, C.A., Olson, E.S., Adams, J.C., Mou, K., Denhardt, D.T., and Davis, R.L. (1995) Osteopontin expression detected in adult cochlea and inner ear fluids.  Hearing Research 85:210-222.

 

156.     Denhardt, D.T., Lopez, C.A., Rollo, E.E., Hwang, S.-m., An, X., and Walther, S.E. (1995) Osteopontin-induced modifications of cellular functions.  In OSTEOPONTIN: ROLE IN CELL SIGNALLING AND ADHESION. Annals of the NY Academy of Sciences  Edited by D. T. Denhardt, W.T. Butler, A.F. Chambers, and D.R. Senger. 760:127-142.

 

157.     Lopez, C.A., Davis, R.L., Mou, K., and Denhardt, D.T. (1995) Activation of a signal transduction pathway by osteopontin. In OSTEOPONTIN: ROLE IN CELL SIGNALLING AND ADHESION. Annals of the NY Academy of Sciences, Edited by D. T. Denhardt, W.T. Butler, A.F. Chambers, and D.R. Senger. 760:324-326.

 

158.     Matsumoto, H.N., Tamura, M., Denhardt, D.T., Obinata, M., and Noda, M. (1995) Establishment and characterization of bone marrow stromal cell lines that support osteoclastogenesis. Endocrinology 136:4084-4091.

 

159.     Feng, B., Rollo, E.E., and Denhardt, D.T. (1995) Osteopontin (OPN) may facilitate metastasis by protecting cells from macrophage NO-mediated cytotoxicity:  Evidence from cell lines down-regulated for OPN expression by a targeted ribozyme.  Clin. Exptl. Metastasis 13:453-462.

 

160.     Malyankar, U.M., Rittling, S.R., and Denhardt, D.T. (1996) Generation of Stable Mitogen Regulated Protein/Proliferin mRNA in Mouse Embryo Fibroblasts Transiently Transfected with Genomic Clones.  J. Cellular Biochemistry 60:198-210.

 

161.          Staal, A., van Wijnen, A.J., Desai, R.K., Pols, H.A.P., Birkenhager, J.C., DeLuca, H., Denhardt, D.T., Stein, J.L., van Leeuwen, J.P.T.M., Lian, J.B., and Stein, G.S. (1996) Antagonistic effects of TGFβ on vitamin D3 enhancement of osteocalcin and osteopontin transcription reduced interactions of VDR/RXR complexes with vitamin D response elements.  Endocrinology 137:2001-2011.

 

162.     Rollo, E. E., Laskin, D.L., and Denhardt, D.T. (1996) Osteopontin inhibits nitric oxide production and cytotoxicity by activated RAW264.7 macrophages.    J. Leucocyte Biol. 60:397-404.

 

163.     Rollo, E.E., and Denhardt, D.T.  (1996) Differential effects of osteopontin on the cytotoxic activity of macrophages from young and old mice.  Immunology 88:642-647.

 

164.     Walther, S.E., and Denhardt, D.T. (1996) Directed mutagenesis reveals that two histidines in tissue inhibitor of metalloproteinase-1 are each essential for the suppression of cell migration, invasion and tumorigenicity. Cell Growth Differentiation 7: 1579-1588.

 

165.     Denhardt, D.T. (1996) Signal transducing protein phosphorylation cascades mediated by RAS/RHO proteins in the mammalian cell:  The potential for multiplex signalling.  Biochemical Journal 318:729-747.

 

166.     Tezuka, K-i., Denhardt, D.T., Rodan, G.A., and Harada, S-i. (1996) Stimulation of mouse osteopontin promoter by v-Src is mediated by a CAAT-box binding factor. J. Biol. Chem. 271: 22713-22717.

 

167.          Denhardt, D.T. (1996) Oncogene-initiated aberrant signalling engenders the metastatic phenotype: Synergistic transcription factor interactions are targets for cancer therapy.  Crit. Revs. Oncogenesis 7:261-291.

 

168.     Brunner, S., Kramar, K., Denhardt, D.T., and Hofbauer, R. (1997) Cloning and characterization of murine carnitine acetyltransferase:  Evidence for a requirement during cell cycle progression. Biochem. J. 322:403-410.

 

169.          Matsue, M., Kageyama, R., Denhardt, D.T., and Noda, M.  (1997) Helix-Loop-Helix Transcription Factor (HES-1) Is Expressed in Osteoblastic Cells, Suppressed by 1,25(OH)2 Vitamin D3 and modulates 1,25(OH)2 Vitamin D3 Enhancement of Osteopontin Expression.   Bone 20: 329-334.

 

170.     Bonnelye, E., Vanacker, J.M., Dittmer, T., Begue, A., Desbiens, X., Denhardt, D.T., Aubin, J., Laudet, V., and Fournier, B. (1997) The ERR-1 orphan receptor is a transcriptional activator expressed during bone development.  Molecular Endocrinology 11:905-916.

 

171.     Rajan, S.S., Denhardt, D.T., Lackland, H., and Ludescher, R.D. (1998) Analysis of the conformational stability of the active domain of recombinant mouse TIMP-1 by intrinsic fluorescence. Biochem. Biophys. Res. Comm. 242:303-309.

 

172.     Rajan, S.S., Lackland, H., Stein, S., and Denhardt, D.T. (1998) Presence of an N-terminal polyhistidine tag facilitates stable expression of an otherwise unstable N-terminal domain of mouse tissue inhibitor of metalloproteinase-1 in E. coli. Protein Expression and Purification 13:67-72.

 

173.  Rittling, S.R., Matsumoto, H., McKee, M., Nanci, A., An, X., Novick, K., Kowalski, A., Noda, M., and Denhardt, D.T. (1998) Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro. Bone and Mineral Research 13:1101-1111.

 

174.     Hoerrner, L.A., Kowalski, A.J., Diamond, J.R., Wilson, P.D., and Denhardt, D.T. (1998) Homeostatic and pathological actions of nitric oxide in the kidney. In Cellular and Molecular Biology of Nitric Oxide, pp 137-169. Edited by J. D. Laskin and D. L. Laskin. Marcel Dekker Inc., NY.

 

175.     Denhardt, D.T., and Noda, M. (1998) Osteopontin expression and function: Its role in bone remodeling. J. Cellular Biochemistry Suppl. 30/31, 92-102.

 

176.     Denhardt, D.T. (1999) Signal transduction pathways and regulation of the mammalian cell cycle: Cell type-dependent integration of external signals. In The Molecular Basis of Cell Cycle and Growth Control  (Editors: G. S. Stein, R. Baserga, A. Giordano, D. T. Denhardt)  J. Wiley & Sons Inc., New York and London.

 

177.     Rittling, S.R., and Denhardt, D.T. (1999) Osteopontin function in pathology: Lessons from OPN-deficient mice. Experimental Nephrology 7, 103-113.

 

178.     Denhardt, D.T. (1999) Coliphage ΦX174 and related phages (Microviridae). In Encyclopedia of Virology 2nd edition. Edited by A. Granoff and R. Webster.  Pp 274-281. Academic Press Ltd, London.

 

179.     Yoshitake, H., Rittling, S.R., Denhardt, D.T., Noda, M. (1999) Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption. Proc. Natl. Acad. Sci. (US) 96, 8156-8160.

 

180.     Denhardt, D.T., Geller, H.M., Kaminski, H.R. (1999) Chemokines. In Encyclopedia of Molecular Biology. Edited by T. E. Creighton, pp 408-413. John Wiley & Sons, New York.

 

181.     Noiri, E., Dickman, K., Miller, F., Romanov, G., Romanov, V.L., Shaw, R., Chambers, A.F., Rittling, S.R., Denhardt, D.T., and Goligorsky, M.S. (1999) Reduced tolerance to acute renal ischemia in mice with a targeted disruption of the osteopontin gene. Kidney International 56:74-82.

 

182.     Ashkar, S., Weber, G.F., Panoutsakopoulou, .V, Sanchirico, M.E., Jansson, M., Zawaideh, S., Rittling, S.R., Denhardt, D.T., Glimcher, M.J., Cantor, H. (2000) Eta‑1 (osteopontin): an early component of type‑1 (cell‑mediated) immunity. Science 287, 860-864.

 

183.  Denhardt, D.T. (2000) On the paradoxical ability of TIMPs either to inhibit or to promote the development and progression of the malignant phenotype.  In “Inhibitors of Metalloproteinases in Development and Disease”. Pp 137-151.  Edited by S.P. Hawkes, D.R. Edwards, and R. Khokha.  Harwood Academic Publ. Lausanne, Switzerland.

 

184.     Tian, J.T., Sørensen, E.S., Butler. W.T., Lopez, C.A., Sy, M.-S., Desai, N.K., and Denhardt, D.T. (2000) Regulation of NO Synthesis Induced by Inflammatory Mediators in RAW264.7 Cells: Collagen Prevents Inhibition by Osteopontin. Cytokine 12:450-457.

 

185.     Bonvini, J.M., Schatzmann, U., Beck-Schimmer, B., Sun, L.K., Rittling, S.R., Denhardt, D.T., Hir, M.L., and Wuthrich, R.P. (2000) Lack of an in vivo function of osteopontin in experimental anti-GBM nephritis. J. Am. Soc. Nephrol. 11:1647-1655.

 

186.     Wu, Y., Denhardt, D.T., and Rittling, S.R. (2000) Osteopontin expression is required for full expression of the transformed phenotype by the Ras oncogene. Brit. J. Cancer. 83:156-173.

 

187.     Asou, Y., Rittling, S.R., Yoshitake, H., Tsuji, K., Shinomiya, K., Nifuji, A., Denhardt, D.T., and Noda, M. (2001) Osteopontin facilitates angiogenesis, accumulation of osteoclasts, and resorption of ectopic bone. Endocrinology 142:1325-1332.

 

188.     Attur, M.G., Dave, M.N., Stuchin, S., Kowalski, A.S., Steiner, G., Abramson, S.B., Denhardt, D.T.,and Amin, A.R. (2001) Osteopontin: An intrinsic inhibitor of inflammation in cartilage. Arthritis and Rheumatism 44:578-584.

 

189.     Ishijimi, M., Rittling, S.R., Yamashita, T., Tsuji, K., Kurosawa, H., Nifuji, A., Denhardt, D.T., and Noda, M. (2001) Enhancement of osteoclastic bone resorption and suppression of osteoblastic bone formation in response to reduced mechanical stress do not occur in the absence of osteopontin. J. Exp. Med. 193:399-404.

 

190.     Denhardt, D.T., Noda, M., O=Regan, A.E., Pavlin, D., and Berman, J.S. (2001) Osteopontin- a means to cope with environmental insults: Regulation of inflammation, tissue remodeling and cell survival. J. Clin. Invest. 107:1055-1061.

 

191.     Nemoto, H., Rittling, S.R., Yoshitake, H., Furuya, K., Amagasa, T., Tsuji, K., Nifuji, A., Denhardt, D.T., Noda, M. (2001) Osteopontin deficiency reduces experimental tumor cell metastasis to bone and soft tissues. J. Bone Mineral Res. 16:652-659.

 

192.     Denhardt, D.T., Giachelli, C.M., Rittling, S.R. (2001) Role of osteopontin in cellular signaling and toxicant injury. Annu. Rev. Pharmacol. Toxicol. 41:721-749.

 

193.     Ihara H, Denhardt DT, Furuya K, Yamashita T, Muguruma Y, Tsuji K, Hruska KA, Higashio K, Enomoto S, Nifuji A, Rittling SR, Noda M. (2001) Parathyroid hormone-induced bone resorption does not occur in the absence of osteopontin. J. Biol. Chem. 276:13065-13071.

 

194.     Denhardt, D.T., Burger, E.H., Kazanecki, C., Krishna, S., Semeins, C.M., and Klein-Nulend, J. (2001) Osteopontin-deficient bone cells are defective in their ability to produce NO in response to pulsatile fluid flow. Biochem. Biophys. Res. Comm. 288: 448-453.

 

195.     Chabas, D.,Baranzini, S.E., Mitchell, D., Bernard, C.C.A., Rittling, S.R., Denhardt, D.T., Sobel, R.A., Lock, C. Karpuj, M, Pedotti, R., Heller, R. Oksenberg, J.R., and Steinman, L. (2001) Osteopontin Influences The Transition from Relapsing / Remitting to Progressive Autoimmune Demyelinating Disease. Science 294:1731-1735.

 

196.     Potter, M.R., Rittling, S.R., Denhardt, D.T., Roper, R.J., Weis, J.J., Teuscher, C., and Weis, J.J. (2001) Role of osteopontin in murine lyme arthritis or host defense against Borrelia burgdorferi. Infection Immunity 70:1372-1381.

 

197.     Noda, M., and Denhardt, D. T. (2002) Osteopontin. In Principles of Bone Biology, 2nd ed. vol 1, 239-250. Academic Press.

 

198.     Ishijima, M, Tsuji, K, Rittling, SR, Yamashita, T, Kurosawa, H, Denhardt, DT, Nifuji, A, Noda M. (2002) Resistance to unloading-induced three-dimensional bone loss in osteopontin-deficient mice.  J Bone Mineral Res. 4:661‑7.

 

199.     D=Alonzo, R.C., Kowalski, A. J., Denhardt, D. T., Nickols, G. A., and Partridge, N. C. (2002) Regulation of collagenase-3 and osteocalcin gene expression by collagen and osteopontin in differentiating MC3T3-E1 cells. J. Biol. Chem. 277: 24788-24798.

 

200.     Yumoto, K., Ishijima, M., Rittling, S.R., Tsuji, K., Tsuchiya, Y., Kon, S., Nifuji, A., Uede, T. Denhardt, D.T., and Noda, M. (2002) Ostopontin deficiency protects joints against destruction in anti-type II collagen antibody-induced arthritis in mice.  Proc. Natl. Acad. Sci. USA 99:4556-4561.

 

201.     Khan, S., Lopez, C.A., Zhang, J., Sørensen, E., and Denhardt, D.T.  (2002) Osteopontin inhibits apoptosis of endothelial cells deprived of growth factors. J. Cellular Biochemistry 85:728-736.

 

202.     Suzuki, K., Zhu, B., Rittling, S.R., Denhardt, D.T., Goldberg, H.A., McCulloch, C.A., Sodek, J (2002) Colocalization of intracellular osteopontin with CD44 is associated with migration, cell fusion, and resorption in osteoclasts. J Bone Miner Res  17:1486‑1497.

 

203.     Denhardt, D.T., Mistretta, D., Chambers, A.F., Krishna, S., Porter J.F., Raghuram, S., Rittling, S.R. (2003) Transcriptional regulation of the osteopontin promoter and the metastatic phenotype: Evidence for a Ras-activated enhancer in the human OPN promoter. Clin. Expt. Metastasis 20:77-84.

 

204.     Shapses, S.A., Cifuentes, M., Spevak, L., Chowdhury, H., Brittingham, J., Boskey, A.L., Denhardt, D.T. (2003)  Osteopontin facilitates bone resorption decreasing bone mineral crystallinity and content during calcium deficiency. Calcified Tissue Int. 73:86-92.

 

205.     Chellaiah MA, Kizer N, Biswas R, Alvarez U, Strauss‑Schoenberger J, Rifas L, Rittling SR, Denhardt DT, Hruska KA.  (2003) Osteopontin Deficiency Produces Osteoclast Dysfunction Due to Reduced CD44 Surface Expression. Mol Biol Cell. 14:173‑89.

 

206.     Kitahara K, Ishijima M, Rittling SR, Tsuji K, Kurosawa H, Nifuji A, Denhardt DT, Noda M. (2003) Osteopontin deficiency induces parathyroid hormone enhancement of cortical bone formation. Endocrinology. 144:2132‑40.

 

207.     Denhardt DT. (2003)  Commentary re: Q‑T. Le et al., Identification of Osteopontin as a Prognostic Marker for Head and Neck Squamous Cell Carcinomas. Clin. Cancer Res., 9:31‑32,

 

208.     Chellaiah MA, Biswas RS, Rittling SR, Denhardt DT, Hruska KA.  (2003) Rho‑dependent Rho kinase activation increases CD44 surface expression and bone resorption in osteoclasts. J Biol Chem. 278:29086‑97.

 

209.     Morinobu M, Ishijima M, Rittling SR, Tsuji K, Yamamoto H, Nifuji A, Denhardt DT, Noda M. (2003) Osteopontin expression in osteoblasts and osteocytes during bone formation under mechanical stress in the calvarial suture in vivo. J Bone Miner Res. 18:1706-15. 

 

210.     Hampel, D.J., Sansome, C., Romanov, V.I., Kowalski, A.J., Denhardt, D.T., and Goligorsky, M.S. (2003) Osteopontin traffic in hypoxic renal epithelial cells. Nephron Exp Nephrol. 94:e66‑76.

 

211.     Zhu. B, Suzuki K, Goldberg HA, Rittling SR, Denhardt, DT, McCulloch CAG, Sodek J. (2003) Osteopontin modulates CD44-dependent chemotaxis of peritoneal macrophages through G-Protein -Coupled Receptors: Evidence of a role for an intracellular form of osteopontin. J Cell Physiol. 198:155-67.

 

212.     Wong YF, Selvanayagam E, Wei N, Porter J, Vittal R, Hu R, Lin Y, Liao J, Shi JW, Cheung TH, Lo KWK, Yim SF, Yip SK, Ngong DT, Siu N, Chan LKY, Chan CD, Kong T, Kutlina E, McKinnon RD, Denhardt DT, Chin KV, Chung TWK. (2003) Expression genomics of cervical cancer: molecular classification and prediction of radiotherapy response by DNA microarray. Clin Cancer Res. 9:5486-92.

 

213.     Porter JF, Shen S, Denhardt DT (2004) Tissue inhibitor of metalloproteinase-1 stimulates proliferation of human cancer cells by inhibiting a metalloproteinase. Brit J Cancer 90:463-70.

 

214.     Weintraub AS, Lin X, Itskovich VV, Aguinaldo JG, Chaplin WF, Denhardt DT, Fayad ZA. (2004)  Prenatal detection of embryo resorption in osteopontin-deficient mice using serial noninvasive magnetic resonance microscopy. Pediatr Res. 55:419-24

 

215.     Denhardt, DT (2004) The Third International Conference on Osteopontin and Related          Proteins. Calcified Tissue Int 74:213-219.

 

216.     SEQ CHAPTER \h \r 1Porter, JF and Denhardt, DT Membrane Receptors and Signal Transduction Pathways in G1: Regulation of Liver Regeneration and T Cell Proliferation.  In Cell Cycle and Growth Control. Edited by GS Stein and AB Pardee. Chapter 4, pp129-146, 2004

 

217.      Morimoto J, Inobe M, Kimura C, Kon S, Diao H, Aoki M, Miyazaki T, Denhardt DT, Rittling S, Uede T. (2004) Osteopontin affects the persistence of beta-glucan-induced hepatic granuloma formation and tissue injury through two distinct mechanisms. Int Immunol. 16:477-88.

 

218.     Matsui Y, Jia N, Okamoto H, Kon S, Onozuka H, Akino M, Liu L, Morimoto J, Rittling SR,             Denhardt D, Kitabatake A, Uede T. (2004)  Role of osteopontin in cardiac fibrosis and            remodeling in angiotensin II-induced cardiac hypertrophy. Hypertension 43:1195-201.

 

219.     Ohyama Y, Nemoto H, Rittling S, Tsuji K, Amagasa T, Denhardt DT, Nifuji A, Noda M. (2004) Osteopontin-deficiency suppresses growth of B16 melanoma cells implanted in bone and osteoclastogenesis in co-cultures. J Bone Miner Res. 19:1706-11.

 

220. Diao H, Kon S, Iwabuchi K, Kimura C, Morimoto J, Ito D, Segawa T, Maeda M, Hamuro J, Nakayama T, Taniguchi M, Yagita H, Van Kaer L, Onoe K, Denhardt D, Rittling S, Uede T. (2004) Osteopontin as a mediator of NKT cell function in T cell-mediated liver diseases. Immunity. 21:539-50.

 

221.  Porter JF, Sharma S, Wilson DL, Kappil MA, Hart RP, Denhardt DT. (2005) Tissue Inhibitor of Metalloproteinases-1 Stimulates Gene Expression in MDA-MB-435 Human Breast Cancer Cells by Means of its Ability to Inhibit Metalloproteinases. Breast Cancer Res Treat. 94:185-93

 

222.      Contractor T, Babiarz B, Kowalski AJ, Rittling SR, Sørensen ES, Denhardt DT (2005) Osteoclasts Resorb Protein-free Mineral (Osteologic™ Discs) Efficiently in the Absence of Osteopontin. In Vivo 19: 335-342.

 

223.     Nilsson SK, Johnston HM, Whitty GA, Williams B, Webb RJ, Denhardt DT, Bertoncello I,   Bendall LJ, Simmons PJ, Haylock DN. (2005) Osteopontin, a key component of the hematopoietic stem cell niche and regulator  of primitive hematopoietic progenitor cells.       Blood  106:1232-9.

 

224.     Rollo EE, Hempson SJ, Bansal A, Tsao E, Habib I, Rittling SR, Denhardt DT, Mackow ER,              Shaw RD. (2005) The cytokine osteopontin modulates the severity of rotavirus diarrhea. J Virol.    79:3509-16.

 

225.   Zhu Y, Denhardt DT, Cao H, Sutphin PD, Koong AC, Giaccia AJ, Le QT. (2005) Hypoxia        upregulates osteopontin expression in NIH-3T3 cells via a Ras-activated enhancer. Oncogene. 24:6555-63.

 

226.   Kondo H, Nifuji A, Takeda S, Ezura Y, Rittling SR, Denhardt DT, Nakashima K, Karsenty G, Noda M. (2005) Unloading induces osteoblastic cell suppression and osteoclastic cell activation to lead to bone loss via sympathetic nervous system. J Biol Chem. 280:30192-200.

 

227.  Vernon HJ, Osborne C, Tzortzaki EG, Yang M, Chen J, Rittling SR, Denhardt DT, Buyske S, Bledsoe SB, Evan AP, Fairbanks L, Simmonds HA, Tischfield JA, Sahota A. (2005) Aprt/Opn double knockout mice: osteopontin is a modifier of kidney stone disease severity. Kidney Int. 68:938-47.

 

228. Denhardt D. (2005) Osteopontin expression correlates with melanoma invasion. J Invest Dermatol. 2005 124:xvi-xviii.

 

229. Khan SA, Cook AC, Kappil M, Günthert U, Chambers AF, Tuck AB, Denhardt DT. (2005) Enhanced cell surface CD44 variant (v6, v9) expression by osteopontin in breast cancer epithelial cells facilitates tumor cell migration: novel post-transcriptional, post-translational regulation. Clin Exp Metastasis 22:663-73.

 

230. Maeno Y, Nakazawa S, Yamamoto N, Shinzato M, Nagashima S, Tanaka K, Sasaki J, Rittling SR, Denhardt DT, Uede T, Taniguchi K. (2006) Osteopontin participates in Th1-mediated host resistance against nonlethal malaria parasite Plasmodium chabaudi chabaudi infection in mice. Infect Immun. 74:2423-7.

 

231. Koyama Y, Rittling SR, Tsuji K, Hino K, Salincarnboriboon R, Yano T, Taketani Y, Nifuji A, Denhardt DT, Noda M.  (2006) Osteopontin deficiency suppresses high phosphate load-induced bone loss via specific modulation of osteoclasts. Endocrinology. 147:3040-9.

 

232. Schroeter M, Zickler P, Denhardt DT, Hartung HP, Jander S. (2006) Increased thalamic neurodegeneration following ischaemic cortical stroke in osteopontin-deficient mice. Brain. 129(Pt 6):1426-37.

 

233. Da Silva AP, Pollett A, Rittling SR, Denhardt DT, Sodek J, Zohar R. (2006) Exacerbated tissue destruction in DSS-induced acute colitis of OPN-null mice is associated with downregulation of TNF-alpha expression and non-programmed cell death. J Cell Physiol. 208:629-39.

 

234. Lai CF, Seshadri V, Huang K, Shao JS, Cai J, Vattikuti R, Schumacher A, Loewy AP, Denhardt DT, Rittling SR, Towler DA. (2006) An osteopontin-NADPH oxidase signaling cascade promotes pro-matrix metalloproteinase 9 activation in aortic mesenchymal cells. Circ Res. 98:1479-89.

 

235. Ishijima M, Ezura Y, Tsuji K, Rittling SR, Kurosawa H, Denhardt DT, Emi M, Nifuji A, Noda M. (2006) Osteopontin is associated with nuclear factor kappaB gene expression during tail-suspension-induced bone loss. Exp Cell Res. 312:3075-83.

 

236. Mori N, Majima T, Iwasaki N, Kon S, Miyakawa K, Kimura C, Tanaka K, Denhardt DT, Rittling S, Minami A, Uede T. (2007) The role of osteopontin in tendon tissue remodeling after denervation-induced mechanical stress deprivation. Matrix Biol. 26:42-53.

 

237. Saika S, Shirai K, Yamanaka O, Miyazaki KI, Okada Y, Kitano A, Flanders KC, Kon S, Uede T, Kao WW, Rittling SR, Denhardt DT, Ohnishi Y. (2007) Loss of osteopontin perturbs the epithelial-mesenchymal transition in an injured mouse lens epithelium. Lab Invest. 87:130-8.

 

238.  Marsh BC, Kerr NC, Isles N, Denhardt DT, Wynick D. (2007) Osteopontin expression and function within the dorsal root ganglion. Neuroreport. 18:153-7.

 

239.  Wung JK, Perry G, Kowalski A, Harris PL, Bishop GM, Trivedi MA, Johnson SC, Smith MA, Denhardt DT, Atwood CS. (2007) Increased expression of the remodeling- and tumorigenic-associated factor osteopontin in pyramidal neurons of the Alzheimer's disease brain. Curr Alzheimer Res. 4:67-72.

 

240. Kavukcuoglu NB, Denhardt DT, Guzelsu N, Mann AB. (2007) Osteopontin deficiency and aging on nanomechanics of mouse bone. J Biomed Mater Res A. 83:136-44.

 

241. Hashimoto M, Sun D, Rittling SR, Denhardt DT, Young W. (2007) Osteopontin-deficient mice exhibit less inflammation, greater tissue damage, and impaired locomotor recovery from spinal cord injury compared with wild-type controls. J Neurosci. 27:3603-11.

 

242. Kato N, Kitahara K, Rittling SR, Nakashima K, Denhardt DT, Kurosawa H, Ezura Y, Noda M. (2007) Osteopontin deficiency enhances anabolic action of EP4 agonist at a sub-optimal dose in bone. J Endocrinol. 193:171-82.

 

243. Ishijima M, Tsuji K, Rittling SR, Yamashita T, Kurosawa H, Denhardt DT, Nifuji A, Ezura Y, Noda M (2007) Osteopontin is required for mechanical stress-dependent signals to bone marrow cells. J Endocrinol. 193:235-43.

 

244. Kitamura M, Iwabuchi K, Kitaichi N, Kon S, Kitamei H, Namba K, Yoshida K, Denhardt DT, Rittling SR, Ohno S, Uede T, Onoe K. (2007) Osteopontin aggravates experimental autoimmune uveoretinitis in mice. J Immunol. 178:6567-72.

 

245. Christensen B, Kazanecki CC, Petersen TE, Rittling SR, Denhardt DT, Sorensen ES. (2007) Cell type-specific post-translational modifications of mouse osteopontin are associated with different adhesive properties. J Biol Chem. 282:19463-72.

 

246.  Wang KX, Shi Y, Denhardt DT. (2007) Osteopontin regulates hindlimb-unloading-induced lymphoid organ atrophy and weight loss by modulating corticosteroid production. Proc Natl Acad Sci U S A. 104:14777-82.

 

247. Kazanecki CC, Uzwiak DJ, Denhardt DT. (2007) Control of osteopontin signaling and function by post-translational phosphorylation and protein folding. J Cell Biochem. 102:912-24.

 

248. Kazanecki CC, Kowalski AJ, Ding T, Rittling SR, Denhardt DT. (2007) Characterization of anti-osteopontin monoclonal antibodies: Binding sensitivity to post-translational modifications. J Cell Biochem. 102:925-35.

 

249.  Chung CJ, Soma K, Rittling SR, Denhardt DT, Hayata T, Nakashima K, Ezura Y, Noda M.  (2008)   OPN deficiency suppresses appearance of odontoclastic cells and resorption of the tooth root induced by experimental force application. J Cell Physiol. 214:614-20.

 

250. Miyazaki K, Okada Y, Yamanaka O, Kitano A, Ikeda K, Kon S, Uede T, Rittling SR, Denhardt DT, Kao WW, Saika S. (2008)  Corneal wound healing in an osteopontin-deficient mouse. Invest Ophthalmol Vis Sci.49:1367-75.

 

251. Ono N, Nakashima K, Rittling SR, Schipani E, Hayata T, Soma K, Denhardt DT, Kronenberg HM, Ezura Y, Noda M. (2008) Osteopontin negatively regulates parathyroid hormone receptor signaling in osteoblasts. J Biol Chem.283:19400-9.

 

252. Borges K, Gearing M, Rittling S, Sorensen ES, Kotloski R, Denhardt DT, Dingledine R. (2008) Characterization of osteopontin expression and function after status epilepticus. Epilepsia. 49:1675-85.

 

253.  Sato I, Yamamoto N, Rittling SR, Denhardt DT, Hino M, Morimoro J, Sakai F, Fujie A, Uede T. (2008) Osteopontin is dispensable for protection against high load systemic fungal infection. Int Immunopharmacol. 8:1441-8.

 

254.  Diao H, Iwabuchi K, Li L, Onoe K, Van Kaer L, Kon S, Saito Y, Morimoto J, Denhardt DT, Rittling S, Uede T. (2008) Osteopontin regulates development and function of invariant natural killer T cells. Proc Natl Acad Sci U S A. 14;105:15884-9.

 

255.  Wang KX, Denhardt DT. (2008) Osteopontin: role in immune regulation and stress responses. Cytokine Growth Factor Rev. 19:333-45.

 

256. Matsui Y, Iwasaki N, Kon S, Takahashi D, Morimoto J, Matsui Y, Denhardt DT, Rittling S, Minami A, Uede T. (2009) Accelerated development of aging-associated and instability-induced osteoarthritis in osteopontin-deficient mice. Arthritis Rheum. 60:2362-71.

 

257. Wang KX, Shi YF, Ron Y, Kazanecki CC, Denhardt DT  (2009) Plasma osteopontin modulates chronic restraint stress-induced thymus atrophy by regulating stress hormones: inhibition by an anti-osteopontin monoclonal antibody. J Immunol.  182:2485-91.

 

258. Chipitsyna G, Gong Q, Anandanadesan R, Alnajar A, Batra SK, Wittel UA, Cullen DM, Akhter MP, Denhardt DT, Yeo CJ, Arafat HA. (2009) Induction of osteopontin expression by nicotine and cigarette smoke in the pancreas and pancreatic ductal adenocarcinoma cells. Int J Cancer. 125:276-85.

 

259. Maeno Y, Shinzato M, Nagashima S, Rittling SR, Denhardt DT, Uede T, Taniguchi K. (2009) Effect of osteopontin on diarrhea duration and innate immunity in suckling mice infected with a murine rotavirus. Viral Immunol. 22:139-44.

 

260. Sullivan J, Blair L, Alnajar A, Aziz T, Ng CY, Chipitsyna G, Gong Q, Witkiewicz A, Weber GF, Denhardt DT, Yeo CJ, Arafat HA. (2009) Expression of a prometastatic splice variant of osteopontin, OPNC, in human pancreatic ductal adenocarcinoma. Surgery. 146:232-40.

 

261. Grassinger J, Haylock DN, Storan MJ, Haines GO, Williams B, Whitty GA, Vinson AR, Be CL, Li S, Sørensen ES, Tam PP, Denhardt DT, Sheppard D, Choong PF, Nilsson SK. (2009) Thrombin-cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with alpha9beta1 and alpha4beta1 integrins. Blood 114:49-59.

 

262. Shimizu S, Okuda N, Kato N, Rittling SR, Shinomiya K, Muneta T, Denhardt DT, Noda M, Tsuji K, Asou Y. (2010) Osteopontin-deficiency impairs wear debris-induced osteolysis via the regulation of cytokine secretion from macrophage. Arthritis Rheum. 2010 Feb 12. [Epub ahead of print]

 

263. Fujita N, Fujita S, Okada Y, Fujita K, Kitano A, Yamanaka O, Miyamoto T, Kon S, Uede T, Rittling SR, Denhardt DT, Saika S. (2010) Impaired angiogenic response in the corneas of mice lacking osteopontin. Invest Ophthalmol Vis Sci. 51:790-4.

 

264. Haders DJ, Kazanecki CC, Denhardt DT, Riman RE.(2010) Crystallographically engineered, hydrothermally crystallized hydroxyapatite films: an in vitro study of bioactivity. J Mater Sci Mater Med.21:1531-42.

 

265. Lazar M, Sullivan J, Chipitsyna G, Aziz T, Salem AF, Gong Q, Witkiewicz A, Denhardt DT, Yeo CJ, Arafat HA (2010) Induction of monocyte chemoattractant protein-1 by nicotine in pancreatic ductal adenocarcinoma cells: role of osteopontin. Surgery 148:298-309.

 

266. Lazar M, Sullivan J, Chipitsyna G, Gong Q, Ng CY, Salem AF, Aziz T, Witkiewicz A,         Denhardt DT, Yeo CJ, Arafat HA. (2010)  Involvement of osteopontin in the matrix-   degrading and proangiogenic changes mediated by nicotine in pancreatic cancer cells. J          Gastrointest Surg. 14:1566-77.

 

267. Baliga SS, Merrill GF, Shinohara ML, Denhardt DT. (2011) Osteopontin Expression during Early Cerebral Ischemia-Reperfusion in Rats: Enhanced Expression in the Right Cortex    Is Suppressed by Acetaminophen. PLoS One. 6:e14568.